If a single bacteriophage infects a single cell, the bacteriophage will reproduce in the cell. The cells will spread through the soft agar until they create a smooth bacterial lawn, making the entire plate cloudy (but with no colonies on top of the agar). The soft agar will solidify, and the cells will grow embedded in the soft agar. coli cells, T2 phage, and melted soft agar, and pour thiis mixture onto a plate. You'll start with a plate containing agar, as usual. To create a smooth lawn for phage plates, you need to have the bacteria growing embedded in the agar, rather than spread on top of the agar. This is very different from the colonies that you've seen so far. A lawn is a dense growth of bacteria, spread evenly over a plate. To see cells that have been killed by the phage, you will need to create a bacterial lawn. You used the antibiotic to wipe out the cells that don't have plasmids, and the ones that survived were the ones you wanted. Up to now, you have used plasmids that give the cells the ability to grow on antibiotic plates. The challenge will be that you need to see where the cells were killed on your plates. In this lab, you'll use bacteriophage T2, a pathogen that will kill E. Working with phages in the lab requires a different plating technique than the one you've used so far in Bio 6B. In this lab, you'll learn to plate with bacteriophage and use PCR to detect phage DNA. A phage’s ability to induce a cell to make huge numbers of copies of a specific DNA molecule can be quite useful to anyone who is trying to clone a gene. Phages are also widely used as tools for molecular biology. Bacteriophages (phages or Φ for short) are abundant in natural ecosystems, and may be one of the main things limiting the world's bacterial populations. Bacteriophages are viruses that infect bacteria.
0 kommentar(er)
